Home / Program / Pre-Conference Workshop / Applications of NGS bioinformatics analysis on tuberculosis epidemiology: a hands-on workshop

Applications of NGS bioinformatics analysis on tuberculosis epidemiology: a hands-on workshop

Workshop Details

Date: April 26 2024
Time: 09:30-16:30
Venue: Taipei International Convention Center
Registration Fee: NTD 3,000 (≒USD 100)
Capacity: 30


The Union-Asia Pacific Region Conference (Union APR)


Population Health Research Center, College of Public Health, National Taiwan University


Hsien-Ho Lin/ National Taiwan University
Jann-Yuan Wang/ Taiwan Society of Tuberculosis and Lung Diseases

Format of the Workshop

  1. Symposium (2hr): speeches and discussion by inviting three speakers
  2. Basic overview and introduction of NGS and bioinformatic analysis + Hands-on session (4hr)
  3. Participants are required to bring their own laptops to the workshop

Learning Objectives

  1. To understand the fundamental knowledge of NGS data
  2. To understand the application and limitations of NGS analysis in tuberculosis epidemiology
  3. To gain hands-on experience in bioinformatics analysis related to tuberculosis epidemiology


In recent years, Next Generation Sequencing (NGS) has rapidly advanced, with Whole Genome Sequencing (WGS) emerging as a potent tool for deciphering microbial epidemiology. Using the genomic information at the finest level of resolution, WGS promises to enhance our understanding about the anticipated resistance pattern and the molecular mechanism of resistance. It can also be used as a powerful genotyping tool to investigate the chain of transmission and the evolution of the microbes over time. With the increasing availability of sequencing and bioinformatics platforms, WGS has become the gold standard of molecular epidemiologic studies for tuberculosis, or has even become routine practice in certain settings.

In light of this, we are organizing a workshop that gathers researchers from various nations to share insights and applications of this burgeoning technology on tuberculosis. Our goal is for participants not only to learn from shared experiences but also to actively immerse themselves in NGS data practices, potentially sowing the seeds for its integration into their future pursuits.

Tentative Agenda

Time Agenda Preliminary Host or Speakers
9:30-10:00 Registration Pre-preparation of hand-on material
10:00-10:10 Opening Hsien-Ho Lin /
National Taiwan University
10:10-11:20 Application of NGS to understand local TB epidemiology
– Taiwan
– Australia
Hsien-Ho Lin/
National Taiwan University
Ted Cohen/ Yale University
Ben Marais/ The University of Sydney
11:30-12:10 Introduction of NGS and Overview of NGS bioinformatics analysis Chongguang Yang/ Sun Yat-sen University
12:10-13:10 Lunch break
13:10-13:40 Linux environment introduction and basic commands Chien-Yueh Lee/ China Medical University
13:40-14:20 NGS Data pre-processing Chien-Yueh Lee/ China Medical University
14:30-15:30 Variant calling, phylogenetic tree and SNP distance calculation Chieh-Yin (Jenny) Wu/ National Taiwan University
15:45-16:10 Identification of resistant-conferring mutations Chieh-Yin (Jenny) Wu/ National Taiwan University
16:10-16:30 Introduction of web tool: MiDsystem Chien-Yueh Lee/ China Medical University